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KMID : 0861019990140010167
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Korea Journal of Herbology
1999 Volume.14 No. 1 p.167 ~ p.179
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Molecular Biological Study of the Effects of Mokyou-Tang on Cell Cycle and Gene Expressions of Human Lung Cancer Cell ( A549 )
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李炯周/Lee, Hyeong-Ju
金相贊/徐富一/金先熙/卞晟僖/Kim, Sang-Chan/Seo, Bu-Il/Kim, Sun-Hee/Byun, Sung-Hui
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Abstract
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This experimental study was performed to investigate the effects of Mokyou-Tang on cell cycle and gene expressions of human lung cancer cell. I examined the cell cycle progression and cell cycle-related gene expression in A549 using a flow cytometry and a quantitative PT-PCR analysis.
The treatment groups, in accordance with concentration of Mokyou-Tang were classified as 3 group(MYT 100㎕/㎖, MYT 500㎕/㎖, MYT 1,000㎕/㎖), and according to treatment time, were classified as 2 group(24hours, 48hours).
The results were summarized as follows;
1. In cell cycle stage, MYT 100㎕/㎖(48hours) and 1000㎕/㎖(48hours) groups were increased as compared with control group, especially 1000㎕/㎖(48hours) group showed eminent increase.
2. In p53 gene, MYT 500㎕/㎖(48hours) and 1000㎕/㎖(48hours) groups showed eminent suppressive effect to cancer cell.
4. CDKNI gene was decreased in all treatment groups, this mean that MYT has suppressive effect to proliferation of cancer cell.
4. In GADD45 gene was decreased in all treatment groups, this mean that MYT has suppressive effect to proliferation of cancer cell.
The results suggest that MYT promotes G1 cell cycle arrest and suppresses the proliferation of cancer cell. Thus, it seems likely that MYT has suppressive effect to proliferation of lung cancer cell.
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KEYWORD
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